Using wblanes is quite straightforward: first load an
image, then select a rectangle from which two bands shall be
quantified.
To load an image, press the Load button which will open a standard
file selector.
Once your image is displayed in the top portion of the software, use
the mouse to draw a rectangle around the region of interest. When the
selected region is defined, the software will perform a regression
analysis to fit a sum of two Gaussians to the bands.
If the fit is successful, a plot and quantitative values will appear
on the lower part of the interface.
The status bar indicates the value
of the area under the first curve A1, under the second curve A2,
and the ratio of the first over the total area in percent (and its
complement under brackets).
The first curve always represents the upper most band in the
original image.
To select a new region of interest, simply drag the existing selection
or draw another rectangle on a different part of the image.
